UA BIOC 471 - ANALYSIS OF PROTEINS AND PROTEOMES (37 pages)

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ANALYSIS OF PROTEINS AND PROTEOMES



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ANALYSIS OF PROTEINS AND PROTEOMES

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Pages:
37
School:
University of Arizona
Course:
Bioc 471 - Ecological Principles for Teachers
Ecological Principles for Teachers Documents

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P1 FUM May 9 2001 14 6 Annual Reviews AR131 14 Annu Rev Biochem 2001 70 437 73 c 2001 by Annual Reviews All rights reserved Copyright ANALYSIS OF PROTEINS AND PROTEOMES BY MASS SPECTROMETRY Matthias Mann 1 2 Ronald C Hendrickson 2 and Akhilesh Pandey3 1Protein Interaction Laboratory and Center for Experimental BioInformatics CEBI Department of Biochemistry and Molecular Biology University of Southern Denmark Campusvej 55 5230 Odense M Denmark e mail mann bmb sdu dk 2MDS Proteomics A S Staermosegaardsvej 6 5230 Odense M Denmark e mail hendrickson protana com 3Whitehead Institute for Biomedical Research 9 Cambridge Center Cambridge Massachusetts 02142 and Brigham and Women s Hospital Boston Massachusetts 02115 e mail pandey bmb sdu dk Key Words functional genomics signal transduction posttranslational modifications protein interaction phosphorylation proteomics Abstract A decade after the discovery of electrospray and matrix assisted laser desorption ionization MALDI methods that finally allowed gentle ionization of large biomolecules mass spectrometry has become a powerful tool in protein analysis and the key technology in the emerging field of proteomics The success of mass spectrometry is driven both by innovative instrumentation designs especially those operating on the time of flight or ion trapping principles and by large scale biochemical strategies which use mass spectrometry to detect the isolated proteins Any human protein can now be identified directly from genome databases on the basis of minimal data derived by mass spectrometry As has already happened in genomics increased automation of sample handling analysis and the interpretation of results will generate an avalanche of qualitative and quantitative proteomic data Protein protein interactions can be analyzed directly by precipitation of a tagged bait followed by mass spectrometric identification of its binding partners By these and similar strategies entire protein complexes signaling pathways and



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