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UIC BIOS 100 - Exam III

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-1-BIOS 100, Cells and Organisms Name:Exam III, 7 November, 2007Michael Muller, InstructorThis exam consists of 53 simple questions gratuitously dispersed over the following seven pages. Last exam I gave you bonus points for bubbling in your last name first, and everyone got them. Woohoo! I was truly happy with this (can you tell, I don’t give out my woohoos lightly). Nowwe’re going to just assume you can do this... but if you don’t, you’ll be penalized your extracredit points, so don’t blow it... Good luck1. Which of the following statements (A-D) about restriction endonucleases is FALSE? Ifstatements A-D are true, then choose E.A. Restriction endonucleases are naturally occurring enzymes found in bacteriaB. Restriction endonucleases cut DNA at specific nucleotide sequencesC. Most restriction sites are not pallindromesD. Restriction endoucleases typically leave sticky ends when they cut DNAE. All of the above statements are TRUE.2. Put the steps to creating a cDNA library that contains the human growth hormone gene in theproper orderI. Use reverse transcriptase to synthesize a cDNA from each mRNAII. Introduce recombinant plasmids into E. coli cells. Grow cells in the presence of anantibiotic - those cells which took in a plasmid will be resistant and then growIII. Isolate mRNAs from cells in pituitary glandIV. Attach restriction endonuclease recognition site to ends of each cDNAV. Cut cDNAs and plasmids with restriction endonucleases - remaining sticky ends joinby complementary base pairing and then ligate cDNA & plasmids with ligaseA. III, II, IV, V, I B. III, I, IV, V, II C. III, IV, V, I, II D. I, III, V, IV, II3. Whose your daddy? Given the gel below to determine who the father isMotherOffspringPotential Father APotential Father BPotential Father C-2-4. Why is the use of Taq polymerase so important in PCR?A . Taq polymerase is a DNA polymerase that can function in the extreme temperatureconditions of PCRB . Taq polymerase is a DNA polymerase that can function in the extreme acidicconditions of PCRC . Taq polymerase is a DNA polymerase that can function in the extreme basicconditions of PCRD . Taq polymerase is a DNA polymerase that can function in the extreme voltagepotential of PCRE. None of the above5. What is a plasmid?A. A piece of DNA containing an antibiotic resistance geneB. A circular ring of DNA which typically contains supplemental DNA in bacteriaC. A piece of DNA containing a restriction siteD. A portion of the main circular loop of naked DNA found in bacteriaE. A protist that can cause diarrhea 6. How are dideoxyribonucleoside triphosphates (ddNTP) used in the Sanger method of DNAsequencing?A. They lack a phosphate group, so they cannot be attached to a growing DNA chainB. They lack a 3' OH group so they terminate DNA replicationC. They glow different colors under ultraviolet light so they can be used to light up eachbaseD. They are not recognized by DNA polymerase III and terminate replicationE. None of the aboveUse the diagrams below to answer questions 7-9 about a 2n=8 cellA B C D 7. Which of the above cells is in metaphase II of meiosis?8. Which of the above cells is in metaphase of mitosis?9. Which of the above cells is in metaphase I of meiosis?10. When do the homologous chromosomes separate during meiosis?A. Metaphase I B. Anaphase I C. Metaphase II D. Anaphase II-3-Use the key below to answer questions 11 - 14A. G0 B. G1 C. G2 D. S E. M11. The chromosomes are doubled during the entire length of which stage of the cell cycle?12. When a cell does not need to divide, it will enter into this non-dividing stage of the cell cycle13. During which stage of the cell cycle are cyclins destroyed?14. Topoisomerase is most active during this stage of the cell cycle15. The product of mitosis is:A. Two genetically identical cells B. Two genetically unique cellsC. Four genetically identical cells D. Four genetically unique cells16. Which of the following statements (A-D) about MPF is FALSE? If statements A-D are true,then choose E.A. MPF is an aggregation of a CDK and a cyclinB. MPF complexes are formed at the G2 checkpointC. MPF levels are greatest during the M phase of MitosisD. MPF initiates a process which destroys cyclins, thus ending mitosisE. All of the above statements are TRUE17. Tumor supressor genes:A. Are genes which will become tumorsB. Are genes which actively destroy tumorsC. Are genes which speed up the rate of cell divisionD. Are genes which slow down the rate of cell divisionE. None of the above18. Which of the following statements (A-D) about non-disjunctions is FALSE? If statementsA-D are true, then choose E.A. Non-disjunctions result when chromosomes do not separate properly during meiosisB. Non-disjunctions can occur in both Anaphase I and Anaphase II of meiosisC. Most fertilizations of human gametes with non-disjunctions are fatal - however, someare notD. Down Syndrome is the result of a non-disjunctionE. All of the above statements about non-disjunctions are TRUE19. What is the function of crossing over?A. To increase genetic diversity in the gametesB. To ensure proper separation during Anaphase IC. To ensure that the tetrads to not separate during Prophase I and Metaphase ID. To reduce the rate of non-disjunctionE. None of the above20. A dislodged heart cell floating now in the blood will undergo mitosis and most likely form atumor due to release from contact inhibitionA. True B. False-4-21. All of the following characteristics are typical of dicots except:A. Floral parts in multiples of 4's and 5's B. Fibrous root system C. Secondary growth D. Net venation in leavesE. Vascular cambium present22. The diagram to the right is an example of aA. Monocot rootB. Dicot rootC. Monocot stemD. Herbaceous dicot stemE. Woody dicot stemUse the key below to answer questions 23-26I. Collenchyma IV. Sieve-tube elements and companion cellsII. Parenchyma V. Tracheids and vesselsIII. Sclerenchyma. 23. This is the most common type of living cell in a xylem rayA. I B. II C. III D. IV E. V24. This/these cells function to support the plantA. I & III B. I, II, III C. I, III, V D. I, II, III, IV E. I, II25. This/these cells are living at functional maturity:A. I only B. I, II C. I, II, III D. I, II, IV E. I, II, V26. This/these cells are involved in translocationA. I B. II C. III D. IV E. V27. The leaf mesophyll and the root cortex are examples


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UIC BIOS 100 - Exam III

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