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SCCC BIO 130 - Study Notes

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Slide 1Slide 2Slide 3Slide 4Slide 5Slide 6Slide 7Slide 8Slide 9Slide 10Slide 11Slide 12Slide 13Slide 14Slide 15Slide 16BIO130 Lab 1 Exercise 3 The MicroscopeParts of the Microscope, Light Adjustment, & DiopterMeasuring Field Diameter Using the Stage Micrometer:1. Insure that left ocular lens is turned fully counterclockwise(Lens should be as tall as it can be)2. Focus on the stage micrometer3. Measure the diameter (widest part) of the field of view using the tiny millimeter ruler of the stage micrometer using only your left eye in the left ocular40X Magnification100X MagnificationLine up the 0.0 line on the edge of the visible field: the zeros will no longer be visibleRead off where the ruler ends on the other side: this one looks like 1.745mm Each scope is a little different400X MagnificationUsing field diameter to determine the size of an object specimen feature or cell:1. Know the field diameter of the objective lens you are looking through. (We measured this)2. Center and focus the object.3. “Guess” how many of that object would fit end to end across the diameter of the field. (Diameter = the widest part of the circle of light you see through the lens)10X objective: diameter measured to be 1860µm1860µmLooks like 2 would fit acrossUsing field diameter to determine the size of an object specimen feature or cell:4. Divide the field diameter by the number of objects you think would fit across end to end to determine the size of one of the objects.10X objective: diameter measured to be 1860µm1860µmLooks like 2 would fit across1860µm ÷ 2 = 930µmNew object:New object:10X objective: diameter measured to be 1860µm1860µmLooks like 4 would fit across1860µm ÷ 4 = 465µmThe green oval is approximately 465µm longWhich lens do I use?The object is a fixed size, regardless of lens you should get the same answer!Use the lens that allows you to see the whole object the best: big enough to guestimate how many fit across the diameter, not too magnified that you cannot see the whole object in the same field.10X objective: diameter measured to be 1860µm1860µmLooks like 4 would fit across1860µm ÷ 4 = 465µm465µm40X objective: diameter measured to be 465µmLooks like 1 would fit across465µm ÷ 1 = 465µmThis object (green oval) is 465µm: you should get approximately the same answer which ever lens you use.Dogfish Placoid ScaleDogfish Placoid Scale4X objective(40X)10X objective(100X)40X objective(400X)Measure one diamondhttp://relentlessthirst.files.wordpress.com/2009/02/dogfish1.jpghttp://www.elasmodiver.com/Sharkive%20images/Spiny-dogfish-070.jpgDinoflagellateDinoflagellate4X objective(40X)10X objective(100X)40X objective(400X)Don’t cut off his tails!Red Tidehttp://www.sciencedaily.com/images/2007/08/070830150106-large.jpghttp://www.pac.dfo-mpo.gc.ca/fm-gp/contamination/Images/Red%20Tide%20-%20Mary%20Mackin%202.jpgHuman Cheek CellHuman Cheek Cell4X objective(40X)10X objective(100X)40X objective(400X)Large irregular shaped cells: look like a ruffled sunny side up eggHuman Blood SmearHuman Blood SmearWBCRBC4X objective(40X)10X objective(100X)40X objective(400X)Tiny red dots, even with the 40X objective: guestimate carefully!Dogfish Placoid ScaleDogfish Placoid ScaleDinoflagellateDinoflagellateHuman Cheek CellHuman Cheek CellHuman Blood SmearHuman Blood


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SCCC BIO 130 - Study Notes

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