APh161 - Lecture 13: Molecular MotorsPowerPoint PresentationRogues Gallery of Motor Action: Rotary MotorsRogues Gallery of Motor Action: TranslocationRogues Gallery of Motor Action: Translational Motor 1Rogues Gallery of Motor Action: Translational Motor 2 - MusclesSlide 7Organellar TransportRogues Gallery of Motor Action: Translational Motor 2Slide 10How We Know: Gliding Motility AssaysDynamics of Molecular MotorsStepping KineticsKinesin DataKinesin Data continuedKinesin Randomness DataWhat do we mean by the states?APh161 - Lecture 13: Molecular APh161 - Lecture 13: Molecular MotorsMotorsRob PhillipsCalifornia Institute of TechnologyMitotic Spindle OrganizationCilia and Flagella Assembly and DynamicsFormation of Golgi and ER NetworksVesicle TransportMolecular MotorsMolecular MotorsRogues Gallery of Motor Action: Rotary Rogues Gallery of Motor Action: Rotary MotorsMotorsShow Berg movieShow Yasuda et al. movieRogues Gallery of Motor Action: Rogues Gallery of Motor Action: TranslocationTranslocationRogues Gallery of Motor Action: Rogues Gallery of Motor Action: Translational Motor 1Translational Motor 1Rogues Gallery of Motor Action: Rogues Gallery of Motor Action: Translational Motor 2 - MusclesTranslational Motor 2 - MusclesSee Hugh Huxley review on websiteRogues Gallery of Motor Action: Rogues Gallery of Motor Action: Translational Motor 2 - MusclesTranslational Motor 2 - MusclesHeuser lab – Washington UniversityOrganellar TransportOrganellar Transport (Hirokawa, Science 1998)Rogues Gallery of Motor Action: Rogues Gallery of Motor Action: Translational Motor 2Translational Motor 2Myosin VSpeed: 350 nm/s ATPase: 5.0 1/sKinesinSpeed: 850 nm/s ATPase: 44.0 1/sDyneinSpeed: -1250 nm/s ATPase: 2.0 1/s**ALL INVOLVED IN VESICLE TRANSPORT**Translational MotorsTranslational MotorsHow We Know: Gliding Motility AssaysHow We Know: Gliding Motility AssaysShow Vale movieDynamics of Molecular MotorsDynamics of Molecular MotorsScience, Vol 300, Issue 5628, 2061-2065, 27 June 2003 - Yildiz et al.Show Gelles, Selvin movieStepping KineticsStepping KineticsSpudich et al., PNAS 2000Kinesin DataKinesin DataFig. 3. Kinesin velocity as a function of [ATP] under external loads, F, fixed by a force clamp. The plots, from the top down, are for F = 0, 1.05, 3.59, 4.60, and 5.63 pN, respectively. Data from Block and colleagues (9): solid curves, N = 2 fits; dashed curves, N = 2 predictions (see text) (Fisher and Kolomeisky, PNAS)Kinesin Data continuedKinesin Data continuedFig. 4. Fits to the data of Block and colleagues (9) (and predictions) for velocity as a function of load for fixed concentrations of ATP. Note the inflection points at low [ATP] and convex profile at saturating [ATP]. (Fisher and Kolomeisky, PNAS)Kinesin Randomness DataKinesin Randomness DataFig. 5. Randomness data from Block and colleagues (9) and theoretical fits (A) as a function of external load, F, at fixed [ATP] (note that the two data points at F 5.7 pN and [ATP] = 2 mM appear separately in Block and coworkers: see figure 4 a and b of ref. 9, respectively) and (B) as a function of [ATP] at fixed loads of, from top down, F = 5.69 pN ( ), 5.35 and 4.60 pN (dashed-line predictions), 3.59 pN ( ), and 1.05 pN ( ).What do we mean by the states?What do we mean by the
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