Fleas and LiceCompound MicroscopePictorial and Dichotomous KeysSlide 4Flea AnatomyOcular bristleSlide 7MallophagaMallophaga AnatomyHeterodoxus spinigerM. stramineus vs. M. gallinaeChelopistes meleagridisGoniodes gigasG. gallinae vs. G. dissimilisLipeuris caponisFelicola subrostrataBovicola crassipesB. bovis vs. B. limbataAnopluraAnoplura AnatomyP. humanus vs. P. pubisHaematopinus eurysternusH. suis vs. H. asiniSolenopotes capillatusLinognathus africanusL. setosus vs. L. pedalisLinognathus vituliVeterinary Entomology 208Spring 2010Magnification•Always start at lowest magnification (lowest power objective)•Move to higher objective as necessary (3-4 stations only)Focus•Adjust width of oculars•Adjust coarse, then fine focus•If on the highest magnification, only adjust the fine focus to avoid breaking slideLight•Use condenser to adjust amount of light (dark vs. glare)•Higher magnifications may require more lightBe sure you read where the key is sending you next, as it can get confusing (particularly with lice)Look at arrow on insect to see what characteristic is being studied•The small arrows in the key indicate the structure referenced in the couplet6 specimens:1. Ctenocephalides felis2. Leptopsylla segnis3. Nosopsyllus fasciatus4. Echidnophaga gallinacea5. Pulex irritans6. Xenopysylla cheopisFleas (Siphonaptera)receptaculum seminisIn front of eyeBelow eye1st couplet: number of combs•We don’t have a 3 comb flea2 comb fleas: • Eye presence/absence will separate these 2 species1 comb flea:•There is only one in our set0 comb fleas:•Head shape separates out Echidnophaga gallinacea•Ocular bristle position separates the other 2 speciesKey12 specimens of Mallophaga:1. Heterodoxus spiniger2. Menacanthus stramineus3. Menopon gallinae4. Chelopistes meleagridis5. Goniodes gigas6. Goniocotes gallinae7. Goniodes dissimilis8. Lipeurus caponis9. Felicola subrostrata10. Bovicola crassipes11. Bovicola bovis12. Bovicola limbata10 specimens of Anoplura:1. Pediculus humanus2. Phthirus pubis3. Haematopinus suis4. Haematopinus asini5. Haematopinus eurysternus6. Solenopotes caplillatus7. Linognathus africanus8. Linognathus setosus9. Linognathus pedalis10. Linognathus
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