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-1-BIOS 100, Cells and Organisms Name:Exam II, 14 October, 2009Michael Muller, InstructorThis exam consists of 43 very simple questions lovingly spread over the following seven pages. For your enjoyment, a codon chart is provided on the last page. Stop right now and make surethat you have bubbled in your last name first. You will lose five points from your exam total ifyou don’t do this. I’m not kidding. Really.The dreaded “what makes what” section. Use the key below to answer questions 1 through 6.I. Glycolysis V. Alcohol FermentationII. Oxidation of Pyruvate VI. Lactic Acid FermentationIII. Krebs Cycle VII. Non-cyclic PhotophosphorylationIV. Oxidative Phosphorylation VIII. Cyclic PhotophosphorylationIX. Calvin-Benson Cycle21. Which of the above processes have a net production of CO ?A. II, III B. II, III, IV C. II, III, VI D. II, III, V, VI E . None of the above22. Which of the above processes produce O ?A. IV only B. VII only C. VIII only D. VII, VIII E. IV, VII, VIII3. Which of the above processes utilize an H+ gradient?A. IV, VII B. VII, VIII C. IV, VII, VIII D. II, IV, VII, VIII4. Which of the above systems convert NADH to NAD+?A. IV only B. V, VI C. IV, V D. IV, V, VI E. III, IV,V, VI5. How many of the above processes have a net production of ATP?A. Three B. Four C. Five D. Six E. Seven6. How many of the above processes have a net production of either NADH or NADPH?A. Three B. Four C. Five D. Six E. Seven7. You place a cell in a solution and the cell shrinks slightly. You take the cell out, place it in anisotonic solution, and it expands back to its previous levels. You then take a salt shakerand put some salt in the solution (enough to change the solute concentration). What willhappen to the cell when you place it back into this now saltier solution?A. It will shrink more than it did when you put it in the solution the first timeB. It will shrink as much as it did when you put it in the solution the first timeC. It will shrink less than it did when you put it in the solution the first timeD. The cell will not shrink at allE. The cell will expand8. Decreasing the amount of osmotically active solutes in a plant central vacuole will__________ the turgor pressure of the plant cellA. Increase B. Decrease C. No effect-2-9. Which of the following statements (A-D) about the Na+/K+ pump is FALSE. If statementsA-D are true, then choose E.A. The Na+/K+ pump requires the hydrolysis of ATP to operateB. The Na+/K+ pump has two configurations, one which can bind to Na+ and the otherwhich can bind to K+C. The Na+/K+ produces an equal charge distribution across the membraneD. The Na+/K+ pump is an example of an integral proteinE. All of the above statements about the Na+/K+ pump are TRUEUse the key below to answer questions 10 & 11.I. Active Transport II. Facilitated Diffusion III. Ion ChannelsIV. Osmosis V. Simple Diffusion10. Which of the above processes do not involve carrier proteins?A. V only B. IV only C. IV, V D. III, IV, V E. I, II, III11. Which of the above can transport molecules against their net concentration gradient?A. VI, V B. III, IV, V C. II, III, IVD. I, II, III, IV, V E. I only12. What were the conclusions of the experiments performed by Frederick Griffith?A. DNA was the molecule of heredityB. The lateral transfer of DNA is responsible for bacterial transformationC. Dead bacteria of one strain can transform living bacteria of another strainD. DNAses prevent transformation of Streptococcus bacteriaE. None of the aboveMatch the group of scientists on the left to the contribution to science on the right.13. Avery, McCarty, MacLeod A. First demonstrated transformation in bacteria B. First described the three-dimensional structure of DNA14. Blackburn, Greider & Szostak C. Illustrated that transformation is retarded by DNAsesD. First described telomerase activity15. Rosalind Franklin E. First published that DNA was an anti-parallel doublehelix16. Which of the nucleotide pairings is not commonly seen in nature?A. A - T B. T - U C. C - G D. All of the above are commonly seen in nature17. Which of the following statements (A-D) about DNA is FALSE? If statements A-D are true,then choose EA. DNA is an anitparallel double helixB. DNA base-pairing rules match up a purine with a pyrimidineC. When forming a phosphodiester bond, the 5' phosphate group of one nucleotide bindsto the 3' -OH group another nucleotideD. A DNA nucleotide is more stable than an RNA nucleotideE. All of the above statements about DNA are TRUE-3-18. Why is the study of telomerase so important in cancer research?A. Many cancers are caused by a non-functioning TEP 1 gene (the gene that makestelomerase) malfunctioningB. Cancer cells have active telomerases, effectively making them immortalC. Cancer cells lack telomeres, which makes them immortalD. Cancer cells have multiple copies of the TEP 1 geneE. None of the above19. Which of the following statements about telomeres and telomerases is FALSE?A. Telomeres are only found in eukaryotes with linear chromosomes - they are not foundin bacteriaB. Telomerase is can synthesize DNA in a 3' to 5' directionC. Telomerase contains a built-in primerD. All of your cells contain telomerase active enzymesE. Telomeres consist of repeating DNA sequences (e.g. TTAGGGTTAGGG....)20. Which of the following statements (A-D) about DNA synthesis is FALSE? If statements A-Dare true, then choose E.A. Ligase is more active in the leading strand than in the lagging strandB. There are several replication origins in a eukaryotic chromosomeC. DNA polymerase III requires a primer or some form of double stranded nucleotidepresent before it can begin DNA replicationD. DNA polymerase III and primase both create nucleotide polymers in a 5' to 3' directionE. All of the above statements about DNA synthesis are TRUE.21. What is the function of topoisomerase?A. Removing RNA primers B. Sealing nicks in DNAC. Unwinding DNA D. Removing supercoilsE. None of the above22. Which pair of tubes represents what Meselson and Stahl observed in the first two generationsof their famous experiment?14/14 14/1515/15A B C D-4-Use the following non-template strand of DNA to answer questions 23-26. *5' C C A A A T G C C A A C T A T A G G G T G A C G C C 3' 23. What is the template strand to this piece of DNA?A. 5' C C A A A T G C C A A C T A T A G G G T G A C G C C 3'B. 3' C C A A A T G C C A


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