Bioengineering 6003 Cellular Electrophysiology & BiophysicsIntroduction 2010 EditionBioengineering 6003 Cellular Electrophysiology & BiophysicsOrganization• Instructors: – Rob MacLeod ([email protected])– Steve Poelzing ([email protected])– John Bridge ([email protected])– Alonso Moreno ([email protected])– Frank Sachse ([email protected])– Mike Sanguinetti ([email protected])– John White ([email protected])– Mark Warren ([email protected])• Web page: – http://www.cvrti.utah.edu/~macleod/be6003• Mailing list:– [email protected]– subscribe be-6003 your_name• Grading: – Homework assignments (short essays, problems, and computer simulations)– Laboratory reportsBioengineering 6003 Cellular Electrophysiology & BiophysicsResource Material• Text (for reference): – Ion Channels of Excitable Membranes by Bertil Hille – The Physiology of Excitable Cells by David Aidley– Heart Physiology by Lionel Opie• Web site– www.cvrti.utah.edu/~macleod/be6003• Notes: – may be available on the web site in pdf format.• Additional references: – see web site• Assigned readings: – distributed in class or via web• Computation: Matlab, see web page for links to tutorialsBioengineering 6003 Cellular Electrophysiology & BiophysicsClass Scheduling9:0010:0011:0012:0013:0014:0015:0016:00Mon. Tues. Wed. Thurs. Fri.M/W 10:45-12:15Bioengineering 6003 Cellular Electrophysiology & BiophysicsLaboratory Exercises I• Ion Channel Lab October 25– Mike Sanguinetti– Techniques:• Glass pipette techniques• Single oocyte recordings• Channel characterization– Identify a channel– 3-4 hours lab time, Space for all students at once• Optical Methods Lab Nov. 22-24– Steve Poelzing & Mark Warren– Techniques:• Chemical and physical basis of optical methods• Instrumentation for optical measurement• Use voltage sensitive dyes to detect membrane potentials– cellular and whole heart components– 4-5 hour lab time, divide into groupsBioengineering 6003 Cellular Electrophysiology & BiophysicsLaboratory Exercises II• Computational Lab Nov. 10-12– Frank Sachse & Rob MacLeod– Simulation of ion channels and cells– Requires a laptop in the class (is this a problem?)– Single lab time (3-4 hours) for whole classBioengineering 6003 Cellular Electrophysiology & BiophysicsGoals of the Course• Develop intermediate level understanding of electrophysiological principles• Apply those principles to cardiac and nervous system cells and membranes• Develop basic understanding of measurement techniquesOur Goals:Your Goals?Bioengineering 6003 Cellular Electrophysiology & BiophysicsOutline of the Course• Mathematical and biophysical basics• Membrane channel structure, transport and electrical behavior• Whole cell structure and behavior• Excitation-contraction coupling • Cell to cell