STARTSTARTFOUNDATIONSFOUNDATIONSHow-to 1How-to 1FORMATIONFORMATIONHow-to 2How-to 2SYSTEMSSYSTEMSPROBLEMSPROBLEMSBIOCHEM GENETICS MOL. BIOSTEM CELLS,CLONINGREC. DNACELL TYPE 3DSTRUCTUREVIRUSESCANCERHUMANDISEASELIFELIFENERVOUSIMMUNESYSTEMSBIOLOGYFUTUREFUTUREGenomicsCell BiologySlide 2 - 7.013 - 3/19/07 Purves Figure 4.2Slide 3 - 7.013 - 3/19/07 Purves Figure 4.3Slide 4 - 7.013 - 3/19/07 Purves Figure 4.2NeuronAxonDendritesNucleusMovie courtesy of Frank Gertler, MITSlide 5 - 7.013 - 3/19/07 Slide 6 - 7.013 - 3/19/07 Slide 7 - 7.013 - 3/19/07 Fibroblast (skin)NeuronsRed Blood CellKeratinocytesSlide 8 - 7.013 - 3/19/07Slide 9 - 7.013 - 3/19/07 Purves Figure 12.14Destinations for Newly Translated ProteinsSlide 10 - 7.013 - 3/19/07 Purves Figure 12.16Modification of ProteinsAequorea victoriaCells expressinggreen fluorescentprotein(GFP)GFP cDNAGFP-GFP cDNAGene X cDNAGFPXProtein X-GFP fusionSlide 11 - 7.013 - 3/19/07 Slide 12 - 7.013 - 3/19/07• Red protein @ the membrane• Nucleus = blue• Green = cytoplasmic proteinpresent in subset of cellsCytoplasm: light green Nucleus: strong staining - protein predominantly localized thereER proteins in sorting processMitochondriaFilamentouscytoskeletalproteinsProtein localization is dynamicCytoplasm--------------> Nuclear“Signal”
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