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UB BIO 201 - Study Notes

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Spring, 2001Final Exam, BIO201B eSpring, 2001No calculators. Every question has one and only one correct answer.1. A nuclear matrix preparation was obtained by the appropriate procedures. After addition of SDS, electron microscopy of this nuclear matrix would be expected to show:A. Nuclear lamin fibersB. Both cytoplasmic and centriolar microtubulesC. A network of many-sized fibersD. No identifiable fibers at allE. Microfilaments only2. Addition (by microinjection) of fluorescent-conjugated tubulin, followed by fluorescence microscopy could be used to identify:A. Existing (non-growing) axonemesB. The plus ends of growing microfilamentsC. Newly replicated centriolesD. Intermediate filamentsE. The minus ends of mitotic spindle microtubules3. One difference between cytoplasmic microtubules and centriolar microtubules is:A. Centriolar microtubules don’t have protofilamentsB. Centriolar microtubules are necessary for nuclear division but cytoplasmic microtubules aren’tC. Cytoplasmic microtubules are singlets but centrioloar microtubules are tripletsD. Centriloar microtubules have gamma tubulin but cytoplasmic microtubules don’tE. The amount of centriolar microtubles doubles during mitotic S-phase but the amount of cytoplasmic microtubules doesn’t change4. In vivo, the core histones (not including the H1 histones) are associated with DNA primarily by:A. Covalent bondsB. Hydrophobic interactionsC. Hydrogen bondsD. Van der Waals interactionsE. Ionic bonds5. If you homogenized a fertilized sea urchin egg at its growth temperature (15oC) and then centrifuged the homogentate at 100,000g for 1 hour at 0 to 4oC, you would expect to see:A. No tubulin in the supernatant and cytoplasmic microtubules in the pelletB. Cytoplasmic microtubules in the supernatant and centriolar microtubules in the pelletC. Centriolar microtubules in the pellet but no cytoplasmic microtubules anywhere D. No tubulin in the supernatant and centriolar microtubules in the pelletE. Tubulin in the supernatant but no microtubules anywhere6. Which of the following is false?A. Absorbance = -log TransmittanceB. One mole of light is 6.02 X 1023 photonsC. The extinction coefficient can be used to accurately determine the concentration at any given absorbance, even in the non-linear portion of a standard curveD. Wavelengths of light are measured in nm/cycleE. As wavelength increases, the frequency (#cycles/sec.) decreases17. One difference between kinesins and dyneins is:A. Only kinesins are glycosylated, dyneins are notB. Dyneins are positively charged at pH=7.0 but kinesins are negatively chargedC. Some functional antibodies can inhibit kinesin activity but those antibodies won’t affect dynein activitiesD. Kinesin related proteins play a role in nuclear division but dyneins don’tE. Dyneins only have two heads but kinesins can have as many as three8. If 10mg of compound X (which has a molecular weight of 100g/mole) were dissolved in 100mlof water, what would the final molar concentration of compound X be?A. 10mMB. 1.0mMC. 1.0MD. 100uME. 10M9. How many milliliters of a 10M stock solution of compound A are needed to make 20 milliliters (final volume) of a 1:5 dilution of compound A?A. 1.0mlB. 0.2mlC. 0.1mlD. 4.0mlE. 5.0ml10. GTP hydrolysis is necessary for:A. Transport of large molecules through nuclear poresB. Addition of tubulin subunits to growing microtubulesC. Chromosomal movements during mitotic prophaseD. Muscle contractionE. Retrograde transport11. When a compound (compound B) loses one or more electrons in a redox couple, it is because that compound (compound B) was:A. Reduced by an oxidizing agentB. Oxidized by a reducing agentC. Reduced by a reducing agentD. Chastised by a travel agent E. Oxidized by an oxidizing agent12. 10-4micromolar equals:A. 4.0uMB. 0.1nMC. 4.0mMD. 0.1mME. 4.0nM13. If a solution has a pH = 3.0 in pure water at 25oC, the hydrogen ion concentration is:A. 10-7MB. 0.003MC. 1.0mMD. 3.0uME. 1.0nM214. For proper proton transport during normal mitochondrial electron transport (not ATP synthesis), which best describes the necessary conditions? Note: I.S. is Intermembrane Space.Source of the Direction of Location of Location of the First electron H+ transport Complex III final electron acceptor A. NAD+ I.S. to matrix inner membrane I.S.B. water matrix to I.S. inner membrane matrixC. NADH I.S. to matrix outer membrane inner membraneD. NADH matrix to I.S. inner membrane matrixE. water matrix to I.S. inner membrane I.S.15. A membrane, which contains an integral membrane glycoprotein, was exposed to each of theconditions below and then centrifuged at 100,000g for 1 hour (still with that condition present). This integral membrane glycoprotein should be:A. In the supernatant when 100mM NaCl is included in the solution usedB. In the supernatant, even when no salts are added (in a low salt solution)C. In the pellet when SDS is included in the solution usedD. In the supernatant when SDS is included in the solution usedE. In the pellet when the solution used contains sucrose at a higher density than the membranes16. Which of the statements is false?A. Photolysis of 2 moles of water produces 4 moles of hydrogen ionsB. When an anion dissolves in pure water, the oxygen of water will hydrogen bond to that anionC. Ubiquinone and dolichol are both lipidsD. 2.0mM NaCl dissociates in pure water at pH=7.0 (25oC) into 2.0mM Na+ and 2.0mM Cl-E. The density of hexane is less than the density of water because hexane floats on top of water.17. The role of SDS in SDS-PAGE is to do all of the following except:A. Solubilize insoluble proteinsB. Give proteins a negative chargeC. Break up protein polymers to solubilize the monomeric subunitsD. Ensure that the main factor determining the migration distance of a protein is its molecular weight E. Stain the proteins so they can be seen on the gel18. Which of the following are produced by animal mitochondria for use by chloroplasts and which are given back to the mitochondria as products of photosynthesis?A. Mitochondria produce amino acids and water for the chloroplasts and they give back carbon dioxide and oxygenB. Mitochondria produce carbon dioxide and water for plants and they give back oxygen andcarbohydratesC. Mitochondria give plants NADH and H+ and the chloroplasts give back NADPH and oxygenD. Mitochondria give carbon dioxide


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