Oxidation and Reduction of HemoglobinReversible “Reduction” of OxyhemoglobinSpectra for Oxy- and Deoxy-HemoglobinOxidation of Oxyhemoglobin to MethemoglobinSpectra for Carbonmonoxy- and Met-HemoglobinRedox Reactions of HemoglobinOxidation and Reduction of HemoglobinCHEM-342 Introduction to BiochemistryReversible “Reduction” of OxyhemoglobinAdd a small amount ofsodium dithionite, Na2S2O4 Stir in the presence of airSpectra for Oxy- and Deoxy-HemoglobinWavelength (nm)OxyHbOxyHbDeoxyHbDeoxyHbOxidation of Oxyhemoglobin to MethemoglobinAdd a small amount ofPotassium ferricyanide, K3[Fe(CN)6] OxyHb MetHbSpectra for Carbonmonoxy- and Met-HemoglobinWavelength (nm)CO-HbMet-HbMet-HbRedox Reactions of HemoglobinOxyHbDeoxyHb MetHb-O2Oxidizing agents [-e-]Reducing agents
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