MICROSCOPE LAB 2StereomicroscopeGREEN RIVER COMMUNITY COLLEGE IDS Unit 1MICROSCOPE LAB 2In the first portion of this lab you will be introduced to the stereomicroscope. In the second portion you will use both a stereomicroscope and a compound microscope to observe and draw various organisms and cells.Stereomicroscope Introduction: The stereoscopic (Dissecting or Binocular) microscope or stereomicroscope has advantages for some types of microscopic work. It consists of two complete microscopes. One for each eye, united to form a single instrument. Eachof these microscopes provides separate and somewhat different images, since the object is viewed from slightly different angles. This binocular feature creates a stereoscopic effect. In our minds we are able to combine and interpret these images sothat we observe the object in three dimensions – length, width, and depth. The stereomicroscope like the compound microscope may be used to view objects by transmitted light. Usually, however, we use the stereomicroscope to look at objects by reflected light. This means that the observer looks at an object as she/he might view a book or her/his own hand. The object is visible because light is reflected from its surface. The stereomicroscope is particularly useful for studying things that are too small to be observed with the unaided eye but too large to be seen as a whole with the compound microscope. The stereomicroscope has a relatively low magnification, 7X to 30X and is used for viewing and manipulating relatively large objects. The stereoscopic microscope is similar to the compound microscope except in the following ways. (1) The depth of field is much greater than with the compound microscope and so you view objects in three dimensions, and (2) the light source can be directed down onto as well as up through an object, so objects that are too thick to transmit light can still be viewed by reflected light. Magnification and Field Diameter SizeExamine your stereomicroscope. On the lab worksheet fill in the table 1 using the techniques and knowledge you applied to the compound microscope.Introductory ObservationsDPM IDS Microscope Lab 2 Instructions (page 1)GREEN RIVER COMMUNITY COLLEGE IDS Unit 1Begin by adjusting your microscope so that it gives the lowest possible magnification. Place a small piece of newspaper in the center of the stage. Using the focusing knobs, move the body of the microscope as close to the stage as possible. While looking into the oculars move the newspaper to the your left. In what direction did the newspaper appear to move? Now move the newspaper away from you. In what direction does it appear to move? How do these observations compare to those you recorded when using the compound microscope? Answer questions 2-5 on your lab worksheet.Wet Mount Drawings - In-Lab Drawings. (Complete in the lab.)You will need to supply your own paper for this portion. For each of your drawings write the name of the type of cell you have drawn. Draw EXACTLY and ONLY for what is called. Label only those structures indicated. Remember to make drawings clear and large!!! Present the drawings in the order given below. Drawings out of order will not be accepted. Staple these to the back of your worksheet.1. Onion Cell: Drawings One and Twoa. On the inner, concave side of a piece of onion, peel of a small piece of theepidermis (skin) with forceps. Place this piece (it should be much smaller than a cover slip) on a slide. Avoid overlapping or wrinkling it. Add 1 or 2 drops of water and a cover slip.b. Examine the onion epidermis under low power or your microscope. Look for cell boundaries. Drawing 1: Draw four-six cells to show the shape and arrangement of the cells. On this drawing include the following label: Onion Cells.c. Place a drop of iodine stain along one edge of the cover slip. Apply a small piece of dry paper towel or tissue to the opposite side of the cover slip. This willpull the stain under the cover slip. Note any changes that occur as the stain spreads across the onion epidermis.d. Drawing 2: Switch to high power and draw a SINGLE Cell. On this drawing include the following labels: Onion Cell, Nucleus, Cytoplasm, Cell wall. In addition estimate the length and width of the cell in micrometers(µm) and addthese to your drawing.2. Zabrina: Drawing 3DPM IDS Microscope Lab 2 Instructions (page 2)GREEN RIVER COMMUNITY COLLEGE IDS Unit 1a. Obtain a small piece of a Zabrina leaf. Place it on a slide and observe it under the scanning or low power of the microscope without using a cover slip or place it on a slide and observe it under the Stereoscopic microscope. b. On the lower surface of the leaf observe and draw two guard cells. On this drawing include the following labels: Zabrina, guard cells (Use your text or abiology text book for reference pictures.) 3. Human Blood: Drawing 4a. Obtain a slide of prepared human blood. Under high power, draw One red blood cell and One white blood cell. On this drawing include the following labels: red blood cell and label the specific type of white blood cell seen. See an Anatomy and Physiology or a biology text for a reference. In addition, estimate the diameter of the red blood cell and include this on your drawing. 4. Trichonympha: Drawing 5Trichonympha lives in a symbiotic relationship with termites. These and other organisms occupy the gut of termites, where they digest wood particles eaten by the insect. Termites lack the enzymes necessary to digest wood and are dependent on Trichonympha to make nutrients in the wood available to them. Trichonympha has become so well adapted to the environment of the termites gut that it cannot surviveoutside of it. Thus to examine a specimen, it is first necessary to separate the Trichonympha from the termite. To obtain a specimen of Trichonympha:a. Place a drop of insect Ringers solution on a clean microscope slide.b. Using forceps or your fingers, transfer a termite into the drop of Ringers.c. Place the slide under the dissecting microscope.d. Place the tips of dissecting needles at either end of the termite and pull in opposite directions.e. Locate the long tube that is the termite’s intestine. Remove all the larger parts of the insect from the slide.f. Using a dissecting needle, mash the intestine to release the Trichonympha.g. Cover your preparation with a clean coverslip.DPM IDS Microscope Lab 2 Instructions (page 3)GREEN RIVER COMMUNITY