PowerPoint PresentationDNA Packing in VirusesHIV RNA Packing???Slide 4Slide 5Electron Microscopy Images of the NucleosomeDissecting the NucleosomeElectron Microscopy of Higher Order StructuresAFM Images of the NucleosomeAtomic-Level Structure of the NucleosomeSlide 11Higher Order StructureMeasurements of Equilibrium AccessibilityThe Role of DNA SequenceNucleosomes Care About PositioningConsequences of Nucleosome PositioningGenome Management and Genome Management and the Nucleosomethe Nucleosome(Segal et al.)DNA Packing in VirusesDNA Packing in VirusesBaker Lab-UCSD Cerritelli et al.Structure of Packed DNA Lander et al.Jiang et al.Genomic material tightly packed in ordered arrangements.These pictures of packaged DNA are a jumping off point for our models of the confined DNA.HIV RNA Packing???HIV RNA Packing???http://student.ccbcmd.edu/courses/bio141/lecguide/unit3/viruses/u2fig3a.html(Turner and Summers)oriCIoriCIIxyProbabilityProbabilityxyReplication origins are confined!Chromosome Geography in VibrioChromosome Geography in Vibrio(Theriot et al.)The Chromosome as a Polymer BlobThe Chromosome as a Polymer Blobb=2PElectron Microscopy Images of the Electron Microscopy Images of the NucleosomeNucleosomeDissecting the NucleosomeDissecting the NucleosomeElectron Microscopy of Higher Order Electron Microscopy of Higher Order StructuresStructuresAFM Images of the NucleosomeAFM Images of the NucleosomeThis image was obtained with purified chromatin fragments from chicken erythroid, using the cryo-AFM. It is seen that all the linker DNA is resolved directly, and the lateral dimensions of the nucleosome are similar to those determined by electron microscopy, and are only slightly greater than that from crystallography. The resolution her eis generally higher than that at room temperature. This was at low salt. The orientation of the nucleosomes appears to be random. With this purification (low salt), linker histones are supposed to be retained.Atomic-Level Structure of the Atomic-Level Structure of the NucleosomeNucleosomeAtomic-Level Structure of the Atomic-Level Structure of the NucleosomeNucleosome(Richmond and Davey, 2003)Higher Order StructureHigher Order StructureMeasurements of Equilibrium Measurements of Equilibrium AccessibilityAccessibility(Anderson and Widom)The Role of DNA SequenceThe Role of DNA Sequence(Cloutier and Widom)Nucleosomes Care About PositioningNucleosomes Care About Positioning(Segal et al.)Consequences of Nucleosome Consequences of Nucleosome PositioningPositioning(Segal et
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