The Development of the The Development of the Operon Operon Concept: What CellsConcept: What CellsEat and When They DieEat and When They Die(Berman et al.)The big idea: there aregenes that control othergenes!Bacterial growth curvesExploring Regulatory DiversityExploring Regulatory DiversityKey point: We can work outthe regulation factor for manyother scenarios includingother looping scenarios.Synergistic ActivationBetter census needed!How Should We Think About RegulationHow Should We Think About RegulationQuantitatively?Quantitatively?Wong, Gladney, and Keasling ‘03Rate Equation Perspective“Thermodynamic Models” –Equilibrium NotionsThe Lambda Switch: The Other HydrogenThe Lambda Switch: The Other HydrogenAtom of Gene RegulationAtom of Gene RegulationRoger HendrixBacteriophage Bacteriophage and Their Genomesand Their Genomeshttp://www.biochem.wisc.edu/inman/empics/0020b.jpgThe Life Cycle of The Life Cycle of Bacteriophage Bacteriophage LambdaLambdaPhysical Consequences of the Tight SqueezePhysical Consequences of the Tight Squeezein the Life Cycle of a in the Life Cycle of a BacteriophageBacteriophageRate of packing: 100bp/sec“Some assembly required”Self-assemblyRate of ejection: ≈ 100 - 1000bp/secConstruct a physicalmodel of theseprocesses.Forceful ejectionA Genetic SwitchA Genetic SwitchThe Lambda GenomeThe Lambda GenomeA More Detailed Example of the A More Detailed Example of the ParrtsParrtsListListThe Lambda Switch: The Lambda Switch: Lysogenic Lysogenic StateStateThe Lambda Switch: The Lambda Switch: Lytic Lytic StateStateDNA Geography of the SwitchDNA Geography of the SwitchBinding of Transcription FactorsBinding of Transcription FactorsSynthetic Genetic SwitchSynthetic Genetic SwitchCollins et al. - see course websiteStable SolutionsStable SolutionsPhase Portrait for the SwitchPhase Portrait for the SwitchSynthetic Genetic SwitchSynthetic Genetic SwitchCollins et al. - see course websiteCoupling of Genes in NetworksCoupling of Genes in
View Full Document
Unlocking...