gJWmUOypY-dsKMSTJE6C8l0kOwxXukBlhsS6yZbNVFv0F-JJeRSCsoSp2mfaCh0SHjy8KMhy1Vs5413n2919ug

SMG-8 and SMG-9, two novel subunits of the SMG-1 complex




7 views

Unformatted text preview:

10.1101/gad.1767209 Access the most recent version at doi: 2009 23: 1091-1105 Genes Dev. Akio Yamashita, Natsuko Izumi, Isao Kashima, et al. nonsense-mediated mRNA decay regulate remodeling of the mRNA surveillance complex during SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, Material Supplemental http://genesdev.cshlp.org/content/suppl/2009/05/08/23.9.1091.DC1.html References http://genesdev.cshlp.org/content/23/9/1091.full.html#related-urls Article cited in: http://genesdev.cshlp.org/content/23/9/1091.full.html#ref-list-1 This article cites 45 articles, 20 of which can be accessed free at: service Email alerting click here top right corner of the article or Receive free email alerts when new articles cite this article - sign up in the box at the http://genesdev.cshlp.org/subscriptions go to: Genes & Development To subscribe to Copyright © 2009 by Cold Spring Harbor Laboratory Press Cold Spring Harbor Laboratory Press on November 23, 2009 - Published by genesdev.cshlp.org Downloaded from SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay Akio Yamashita,1,2,8,12 Natsuko Izumi,1,8 Isao Kashima,1,9 Tetsuo Ohnishi,1,10 Bonnie Saari,3 Yukiko Katsuhata,1,4 Reiko Muramatsu,1,2 Tomoko Morita,1 Akihiro Iwamatsu,5 Takahisa Hachiya,6 Rie Kurata,1 Hisashi Hirano,7 Philip Anderson,3 and Shigeo Ohno1,11 1Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama 236-0004, Japan; 2Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency, Kawaguchi 332-0012, Japan; 3Department of Genetics, University of Wisconsin at Madison, Madison, Wisconsin 53706, USA; 4Department of Obstetrics and Gynecology, Yokohama City University School of Medicine, Yokohama 236-0004, Japan; 5Protein Research Network Co. Ltd., Yokohama 236-0004, Japan; 6Ina Laboratory, Medical and Biological Laboratories Co. Ltd., Ina 396-0022, Japan; 7International Graduate School of Arts and Sciences, Yokohama City University, Yokohama 230-0045, Japan Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism that detects and degrades mRNAs containing premature translation termination codons (PTCs). SMG-1 and Upf1 transiently form a surveillance complex termed ‘‘SURF’’ that includes eRF1 and eRF3 on post-spliced mRNAs during recognition of PTC. If an exon junction complex (EJC) exists downstream from the SURF complex, SMG-1 phosphorylates Upf1, the step that is a rate-limiting for NMD. We provide evidence of an association between the SURF complex and the ribosome in association with mRNPs, and we suggest that the SURF complex functions as a translation termination complex during NMD. We identified SMG-8 and SMG-9 as novel subunits of the SMG-1 complex. SMG-8 and SMG-9 suppress SMG-1 kinase activity in the isolated SMG-1 complex and are involved in NMD in both mammals and nematodes. SMG-8 recruits SMG-1 to the mRNA surveillance complex, and inactivation of SMG-8 induces accumulation of a ribosome:Upf1:eRF1:eRF3:EJC complex on mRNP, which physically bridges the ribosome and EJC through eRF1, eRF3, and Upf1. These results not only reveal the regulatory mechanism of SMG-1 kinase but also reveal the sequential remodeling of the ribosome:SURF complex to the predicted DECID (DECay InDucing) complex, a ribosome:SURF:EJC complex, as a mechanism of in vivo PTC discrimination. [Keywords: NMD; mRNA surveillance; UPF1; SMG-1; PIKK; translation termination; mRNP remodeling] Supplemental material is available at http://www.genesdev.org. Received November 25, 2008; revised version accepted March 23, 2009. Translation termination involves recognition of a stop ...





Loading Unlocking...

Login

Join to view SMG-8 and SMG-9, two novel subunits of the SMG-1 complex and access 3M+ class-specific study document.

or
We will never post anything without your permission.
Don't have an account?

Sign Up

Join to view SMG-8 and SMG-9, two novel subunits of the SMG-1 complex and access 3M+ class-specific study document.

or

By creating an account you agree to our Privacy Policy and Terms Of Use

Already a member?