WRITE YOUR NAME AND I.D. NUMBER LEGIBLY ON EVERY PAGE – PAGES WILL BE SEPARATED FOR GRADING! CHECK TO BE SURE YOU HAVE 7 PAGES, NAME (print): INCLUDING COVER PAGE. 5-digit course ID # I swear/affirm that I have neither given nor received any assistance with this exam. Signature: Date: BIOCHEMISTRY 460 FOURTH HOUR EXAMINATION FORM A ANSWERS May 10, 2006 NO CALCULATORS ARE NEEDED OR PERMITTED ON THIS EXAM. NO CELL PHONES MAY BE OUT. (TURN OFF AND PUT ENTIRELY AWAY!) NO TALKING! CHECK SCREEN FROM TIME TO TIME FOR CORRECTIONS, CURRENT TIME, ETC. IF NECESSARY, STAFF WILL REMAIN FOR UP TO 1 ½ HOURS, BUT 50 MIN. SHOULD BE AMPLE TIME. p. 2 (13 points) p. 3 (15 points) p. 4 (17 points) p. 5 (22 points) p. 6 (16 points) p. 7 (17 points) TOTAL: (100 points)Biochemistry 460, Exam #3 Form A ANSWERS NAME April 10, 2006 5-digit course ID# page 2 1. (13 pts) Fill in the table below from the following choices. Some compounds may be used once as an answer, more than once or not at all. Some questions may have more than one correct answer. Put only one answer. If you put more than one answer, you will NOT get credit for the question. Only use compounds from this list. If you put something not included in this list, you will NOT get credit for the question. All questions do have a correct answer. Glucose 6-phosphate Glucose 1-phosphate Glucose 1,6-bisphosphate Fructose 6-phosphate Fructose 1,6-bisphosphate Ribulose 5-phosphate Ribulose 1,5-bisphosphate Erythrose 4-phosphate Glyceraldehyde-3-phosphate Glycerol ADP-Glucose UDP-Glucose Glycogen Cellulose Cellobiose Tyrosine Epinephrine Tryptophan Pectin Starch Lactate Lactose Ethanol Carbon dioxide (CO2) Oxaloacetate (OAA) Fumarate Argininosuccinate Carbamoyl Phosphate Succinate Citrate Description Compound Substrate for glycogen phosphorylase Glycogen Product of glycogen phosphorylase Glucose 1-phosphate Substrate for glycogen synthase UDP-Glucose Product of glycogen synthase Glycogen Substrate for First Enzyme in oxidative portion of the Pentose Phosphate Pathway Glucose 6-phosphate Product of Last Enzyme in oxidative portion of the Pentose Phosphate Pathway Ribulose 5-phosphate Intermediate from the carbon shuffling portion of the Pentose Phosphate Pathway that is a precursor for aromatic amino acids Erythrose 4-phosphate Substrate of Rubisco Ribulose 1,5-bisphosphate OR Carbon Dioxide (CO2) Product of Fermentation in Muscle Cells Lactate Compound that connects the urea cycle to the citrate cycle (is involved in both pathways) Oxaloacetate (OAA) OR Fumarate Substrate for sucrose phosphate synthase UDP-Glucose OR Fructose 6-phosphate Product of pancreatic lipase Glycerol Product of phenylalanine hydroxylase Tyrosine ___/13Biochemistry 460, Exam #3 Form A ANSWERS NAME April 10, 2006 5-digit course ID# page 3 2. (3 pts) Which amino acids are products of the shikimate pathway? A. lysine, serine, arginine B. methionine, leucine, valine C. aspartate, methionine, threonine D. phenylalanine, tryptophan, tyrosine E. phenylalanine, tyrosine, histidine F. glutamine, proline, arginine G. alanine, valine, leucine H. histidine 3. (3 pts) Which amino acids are produced from oxaloacetate? A. lysine, serine, arginine B. methionine, leucine, valine C. aspartate, methionine, threonine D. phenylalanine, tryptophan, tyrosine E. phenylalanine, tyrosine, histidine F. glutamine, proline, arginine G. alanine, valine, leucine H. histidine 4. (3 pts) There are two nitrogen atoms eliminated from the human body in the urea molecule. One of these comes from ammonium ion through carbamoyl phosphate. What compound brings the other nitrogen directly to the urea cycle? A. Aspartate B. Asparagine C. Glutamate D. Glutamine E. Methionine F. Lysine 5. (6 pts) A. (3 pts) What enzyme is inhibited by RoundUp (glyphosate)? (Circle number of correct answer.) 1. DAHP Synthase 2. ATP Synthase 3. Succinate Dehydrogenase 4. Sucrose Phosphate Synthase 5. PEP Carboxylase 6. Nitrogenase 7. EPSP Synthase 8. Cellulose Synthase B. (3 pts) Why does RoundUp (glyphosate) kill plants but not people? (Circle number of correct answer.) 1. Both plants and people have the enzyme inhibited by glyphosate (part A), but only the plant form of the enzyme is sensitive to glyphosate. 2. There is an enzyme in the human liver that detoxifies glyphosate so that people can secrete it in their urine, protecting them from the harmful effects it might have on their metabolism. 3. People do not have the enzyme inhibited by glyphosate (part A), so they are not affected by glyphosate like plants are. 4. People have another pathway in the liver that bypasses the pathway blocked by glyphosate. ___/15Biochemistry 460, Exam #3 Form A ANSWERS NAME April 10, 2006 5-digit course ID# page 4 6. (12 pts) It is not a good idea for a marathon runner to eat a candy bar about 20 – 30 min before the start of a race. Why is this so? Answer the following questions to help explain this phenomenon. A. (2 pts) Intake of a high sugar food will induce production of ___insulin_________ (name hormone) B. (2 pts, circle correct underlined word from each pair) Binding of this hormone to liver cell receptors will activate a signaling pathway that will lead to phosphorylation / dephosphorylation and activation / deactivation of glycogen synthase. C. (2 pts, circle correct underlined word from each pair) Binding of this hormone to liver cell receptors will activate a signaling pathway that will lead to phosphorylation / dephosphorylation and activation / deactivation of glycogen phosphorylase. D. (2 pts, circle number of correct response) The net effect on liver cells of eating the candy bar will be: 1. an uptake of glucose from the blood and storage of glucose in the form of glycogen in the liver cells. 2. a breakdown of glycogen in the liver cells and release of glucose to the blood. E. (2 pts, circle number of correct response) Binding of the hormone (part A above) to the adipose cell receptors will also affect fatty acid metabolism. It will lead to: 1. breakdown of triacylglycerides in adipose cells and secretion of fatty acids and glycerol 2. conversion of glucose and fatty acids into triacylglycerols in adipose cells F. (2 pts, circle correct underlined word) The overall net effect of eating the candy bar is that there will be
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