RESPIRATORY TRACT




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CLINICAL MICROBIOLOGY SERVICE VIRUS HUNTERS RESPIRATORY TRACT DETECTIVES DR PHYLLIS DELLA LATTA VIRUSES THE LOW DOWN OBLIGATE INTRACELLULAR ORGANISMS REQUIRE METABOLICALLY ACTIVE CELLS FOR EFFICENT REPLICATION THERE IS NO UNIVERSAL CELL LINE CERTAIN VIRUSES CANNOT BE CULTURED IN TRADITIONAL CELL CULTURE MONOLAYERS 9 ROTAVIRUS 9 METAPNEUMOVIRUS 9 HEPATITIS PREDICTING THE PATHOGEN AGE 9 PEDIATRICS 9 ADULTS SEASON 9 ENTEROVIRUSES SUMMER FALL 9 INFLUENZA RSV WINTER 9 ROTAVIRUS WINTER SPRING IMMUNE STATUS GEOGRAPHY SKIN 9 HSV VZV ENTERO EYE 9 ADENO HSV VZV ENTERO CNS 9 ENTERO HSV CMV VZV GI 9 ROTA ADENO ENTERO GENITAL 9 HSV LEARNING OBJECTIVES Be familiar with viruses commonly associated with adult and pediatric patients Discuss methodologies available for the rapid detection of viral pathogens Distinguish common causes of community versus hospital associated pneumonia VIROLOGY TESTING BUSTING THE MYTHS THE MYTHS TAT CAN RANGE FROM TURN AROUND MINUTES TO 72 HRS TIME TAT IS TOO 9 DIRECT AG LONG DETECTION 9 MOLECULAR CAN T TREAT A ASSAYS VIRUS MANY ANTI VIRAL ACADEMIC AGENTS AVAILABLE PURSUIT CLINICALLY RELEVANT INFORMATION MINIMAL IMPACT ON PATIENT CARE RESPIRATORY VIRUSES CLINICAL SYNDROMES COMMON COLD 9 RHINO CORONA ENTERO PARA 1 3 ADENO PHARYNGITIS 9 ENTERO ADENO EBV HSV CROUP 9 PARA 1 2 RSV BRONCHIOLITIS 9 RSV PARA 3 INFLU A B PNEUMONIA 9 RSV PARA 1 3 INFLU A B ADENO CMV VZV 1 VIRAL CULTURE MENU OF METHODOLOGIES ROUTINE CULTURE SHELL VIAL CULTURE more rapid RAPID ANTIGEN DETECTION TURNAROUND TIMES 9ROUTINE CULTURE 9 Enzyme Immunoassay EIA INCUBATE FOR UP TO 6 WEEKS AVERAGE TAT HSV RSV INFLUENZA A B ADENO 40 41 ROTAVIRUS 9 Direct Fluorescent Antibody DFA 5 TO 10 DAYS CMV HSV VZV INFLUENZA A B PARAINFLUENZA 1 3 RSV ADENO 9SHELL VIAL CULTURE NUCLEIC ACID AMPLIFICATION SPECIMENS ARE CENTRIFUGED ONTO CELLS TO FACILITATE RECEPTOR BINDING RESULTS IN 1 5 DAYS 9 REAL TIME POLYMERASE CHAIN REACTION PCR CUMC TEST METHODS TAT VIRUS EIA ADENO 40 41 2 HRS DFA SHELL CULTURE VIAL 72






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