Biochemistry 151: Structure and Mechanisms in Membrane Transport ProteinsInstructor – Chris MillerThis is an advanced-topics seminar course that will meet Mondays, 2-5PM. It will be conductedexclusively with primary literature readings. Each week will be aimed at a particular topic in thisfield. Papers for each week’s discussion will be posted several weeks in advance. PDFs of thesepapers will be available on the Brandeis CT course website. Course webpageAll class materials, except for PDF files are available on an open webpage athttp://www.bio.brandeis.edu/classes/biochem151a/Literature readingsAll students are expected to be familiar with all the assigned readings and to present variousaspects of these readings when called upon to do so. Readings will not be assigned each week toparticular students for presentation; instead, all students must come to class prepared to presentand to discuss the readings. PDFs can be downloaded at http://webct.brandeis.edu.Molecular graphicsNearly all the topics to be discussed will have associated with them high-resolution crystalstructures. Students must obtain the pdb files of these structures from an appropriate databaseand view these while reading papers about the proteins. A superb database devoted solely tomembrane protein structures has been constructed by Steve White at UC Irvine:http://blanco.biomol.uci.edu/Membrane_Proteins_xtal.htmlThere are many molecular graphics programs available. I use RasMol because of its simplicity. This program is installed on the Brandeis library computers and may be downloaded from theweb for free: http://www.rasmol.org/I have written a stripped-down RasMol tutorial, which you can access on the course webpage.Schedule of Class Meetings Monday, 2-5 PM, Kosow 116Sep 8 Organization, lecture on basic principles of membrane transportSep 15 Structure of lipid bilayer membranes, membrane-peptide interactionSep 22 NO CLASSSep 29 Architecture and folding thermodynamics of "-helical integral membrane proteinsOct 7 (Tues!) $-barrel proteins. Porins as molecular sievesOct 13 AquaporinsOct 20 P-type ATPase pumpsOct 27 ABC transporters. Multidrug resistance and metal detox transportNov 3 Secondary coupled transport. MFS proteins. LacY, GlpTNov 10 K+ channels – ion permeation and selectivityNov 17 K+ channels – voltage-dependent gatingNov 24 Some exotic transport proteins. TolC, decarb-pumps,