View Full Document

The Molecular Architecture of Galactose Mutarotase/UDP-Galactose



View the full content.
View Full Document
View Full Document

14 views

Unformatted text preview:

THE JOURNAL OF BIOLOGICAL CHEMISTRY 2005 by The American Society for Biochemistry and Molecular Biology Inc Vol 280 No 23 Issue of June 10 pp 21900 21907 2005 Printed in U S A The Molecular Architecture of Galactose Mutarotase UDP Galactose 4 Epimerase from Saccharomyces cerevisiae Received for publication March 3 2005 and in revised form March 15 2005 Published JBC Papers in Press March 28 2005 DOI 10 1074 jbc M502411200 James B Thoden and Hazel M Holden From the Department of Biochemistry University of Wisconsin Madison Wisconsin 53706 D Galactose is converted to the more metabolically useful glucose 1 phosphate by the action of four enzymes that constitute the Leloir pathway as shown in Scheme 1 reviewed in Ref 1 Mutations in the genes that encode for three of these enzymes in humans specifically galactokinase galactose 1 phosphate uridylyltransferase and UDP galactose 4 epimerase result in the diseased state referred to collectively as galactosemia 2 4 The clinical severity of these disorders varies widely depending upon the gene affected and the resulting degree of enzyme impairment Common symptoms include cataract formation impaired cognitive development and liver dysfunction among others In the first step of the Leloir pathway D galactose is converted to D galactose by galactose mutarotase The structures This work was supported by National Institutes of Health Grant DK47814 to H M H The costs of publication of this article were defrayed in part by the payment of page charges This article must therefore be hereby marked advertisement in accordance with 18 U S C Section 1734 solely to indicate this fact The atomic coordinates and structure factors code 1Z45 have been deposited in the Protein Data Bank Research Collaboratory for Structural Bioinformatics Rutgers University New Brunswick NJ http www rcsb org To whom correspondence should be addressed Tel 608 262 4988 Fax 608 262 1319 E mail Hazel Holden biochem wisc edu of the mutarotases from both Lactococcus



Access the best Study Guides, Lecture Notes and Practice Exams

Loading Unlocking...
Login

Join to view The Molecular Architecture of Galactose Mutarotase/UDP-Galactose and access 3M+ class-specific study document.

or
We will never post anything without your permission.
Don't have an account?
Sign Up

Join to view The Molecular Architecture of Galactose Mutarotase/UDP-Galactose and access 3M+ class-specific study document.

or

By creating an account you agree to our Privacy Policy and Terms Of Use

Already a member?