CU-Boulder CHEM 5181 - MALDI Software Users Guide (5 pages)

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MALDI Software Users Guide



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MALDI Software Users Guide

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Pages:
5
School:
University of Colorado at Boulder
Course:
Chem 5181 - Mass Spectrometry and Chromatography

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8 23 2010 MALDI Software Users Guide simplified version Central Analytical Lab Ekeley E266 The Acquisition program is called Voyager Control Panel Once you shoot your sample and determine it looks good you transfer the data to Data Explorer the Analysis software tool I To Begin Acquisition i Starting the Software The oscilloscope and video monitor must be ON before opening the software They are supposed to be ON all the time When turning on the scope wait for a moment until the scope gets to the initial screen The software is located on the desktop open Voyager Control Panel ii Load a Sample Plate use a flat plate with no deep wells Go to Hand Icon on the tool bar Eject Autosampler will take a few moments to eject the plate holder Be very careful when hooking up a sample plate Never put a wet plate or samples in Go to the Hand Icon again Plate ID 100 wells Load If you re finished for the day select the Load No Plate option to get the empty plate holder back in Also get the holder back in when you temporarily leave the instrument Leaving the holder out for an extended period of time is not good for the vacuum system of the instrument iii Open a Method File called Instrument Settings Prior to the measurement you must open an appropriate Method File in your Settings folder To open an Instrument Setting bic file under File select Open Instrument Settings You can only modify and save changes to your own instrument setting files use disk button top left to save Your location and Method Files on the computer D MALDI data Your sub directory Data Settings Linear meth pos bic Linear meth pos over10kDa bic Reflector meth pos bic Page 1 of 5 8 23 2010 iv Load a Mass Calibration File see II v below II Control Panel Settings i File Naming and Saving This is located on the upper left hand side of the Control Panel Window Choose your directory in the gray box by selecting the Browse button All data should be saved to the Data folder in Your location Below this is a box for your



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