PowerPoint PresentationSlide 2Slide 3Slide 4Slide 5Slide 6Slide 7Slide 8Slide 9Slide 10Slide 11Slide 12Slide 13AUGUAAOpen Reading Frame(ORF)Generic Structure of a Eukaryotic mRNA(AAA)n(AUUUA)n7-Me-GpppG5’-untranslated region (5’-UTR)3’-untranslated region (3’-UTR)cappoly(A) tailcleavage& poly(A)addition sitestem-loop structuresstem-loop structures-6 -5 -4 -3 -2 -1 +4 G C C A C C G-6 -5 -4 -3 -2 -1 +4(GC-poor) A x A (U)Kozak (mammalian) consensus:S. cerevisiae (yeast) consensus: Initiation codon context7-methyl-guanineR1 and R2 = H or CH3mRNA-3’-OH• ATP and GTP • GTPGcn2 &other eIF2kinasesMammalian eIF4G1eIF4EComposedof 13 distinctgene products (eIF3a-eIF3m)eIF3eIF4GmRNA40S subunit40S subuniteIF3Translation Factor Yeast Gene Name Size (residues) Function / CommentseIF4E CDC33 213 7MeG cap-binding proteineIF4E-BP1 CAF20 161 Cdc33-binding proteineIF4E-BP2 EAP1 632 Cdc33-binding proteineIF4G1 TIF4631 952 Scaffold protein, isoform 1eIF4G2 TIF4632 914 Scaffold protein, isoform 2eIF4A1 TIF1 395 RNA-dependent RNA helicase, isoform 1eIF4A2 TIF2 395 RNA-dependent RNA helicase, isoform 2eIF4B TIF3 436 RNA-binding protein, stimulates eIF4APAB PAB1 577 Poly(A)-binding proteineIF3a TIF32 / RPG1 964 p110 subunit of eIF3eIF3b PRT1 763 p90 subunit of eIF3, RNA-binding proteineIF3c NIP1 812 p93 subunit of eIF3, required for nucleocytoplasmic transport of eIF3eIF3g TIF35 274 p33 subunit of eIF3, RNA-binding proteineIF3i TIF34 347 p39 subunit of eIF3, two WD40 repeatseIF3j HCR1 265 p29 subunit of eIF3, binds to A site and mRNA entry channel of 40S eIF3 ? SUI1 108 p16 subunit of eIF3, involved in initiation codon recognitioneIF3 ? TIF31 / CLU1 964 p135 subunit of eIF3, role in scanning for AUGeIF3 ? GCD10 478 p62 subunit of eIF3, RNA-binding proteineIF3 ? GCD14 383 tRNA 1-methyladenosine methyltransferase, when associated with
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