PhilaU CHEM 201L - Analysis of Analgesic Tablets by Thin Layer Chromatography (19 pages)

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Analysis of Analgesic Tablets by Thin Layer Chromatography



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Analysis of Analgesic Tablets by Thin Layer Chromatography

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Pages:
19
School:
Philadelphia University
Course:
Chem 201l - Organic Chemistry I Laboratory

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Analysis of Analgesic Tablets by Thin Layer Chromatography TLC Organic Chemistry Lab I Fall 2008 Dr Milkevitch Oct 12 14 2009 Separating Molecules Today Learn a separation technique Molecules can be separated by a number of methods Extraction Precipitation Chromatography Chromatography to write in color Originally developed to separate components of inks Thin Layer Chromatography Thin Layer Chromatography The separation of moderately volatile or nonvolatile substances based upon differential adsorption on an inert solid stationary phase immersed in a organic solvent or solvent mixture mobile phase Makes use of A stationary phase solid support Mobile phase a fluid Basic principle of separation Differential interactions of molecules with these 2 phases How the separation works Molecules can move with the mobile phase Or they can remain fixed on the stationary phase Called partitioning How molecules partition between the 2 phases depends on Properties of the phases Properties of the molecules being separated How this works continued Molecules that partition into the mobile phase Will move more rapidly than molecules that partition into the stationary phase Therefore they will separate from those that are partitioned into the stationary phase Faster moving component partitioned more into mobile phase Slower moving component partitioned more into stationary phase Solvent movement Two component mixture applied to TLC plate Nuts and Bolts of the Method You must carefully select mobile and stationary phases to achieve full separation of mixtures of molecule Trial and error Stationary phase thin layer of silica spread on a surface Mobile phase solvent system that migrates through the silica Samples are spotted on the plate Moved along by the solvent s migrating through the silica Rf Values Molecules that are separated will migrate as spots and their migration can be measured Migration usually reported as an Rf value Calculation of an Rf value Ratio of sample migration how



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