CU-Boulder CHEM 4761 - INTRODUCTION TO NUCLEIC ACIDS AND MOLECULAR CLONING (60 pages)

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INTRODUCTION TO NUCLEIC ACIDS AND MOLECULAR CLONING



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INTRODUCTION TO NUCLEIC ACIDS AND MOLECULAR CLONING

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Pages:
60
School:
University of Colorado at Boulder
Course:
Chem 4761 - Biochemistry Laboratory

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INTRODUCTION TO NUCLEIC ACIDS AND MOLECULAR CLONING References E coli Cellular and Molecular Biology F C Neidhardt ed Volumes 1 2 1987 a detailed reference on things E colial www slic2 wsu edu 82 hurlbert micro101 pages 101hmpg ht ml an excellent review of most of the things we will do in the last section of the lab A MUST READ BEFORE DOING ANEXPERIMENT website Voet Biochemistry pp916 918 932 933 897 902 Jacobs Life in the Balance Cell Walls Science 278 1731 1997 The next series of experiments will be devoted to the nucleic acids In them se we are going to learn some of the basic techniques that are used by biochemists who study nucleic acids At one time nucleic acid research was a separate branch of the field of biochemistry practiced mainly by microbiologists and a few hardy organic chemists and physicists Researchers could and did study enzymes without knowing much about how to work with nucleic acids except perhaps how to get rid of them in the early steps of enzyme purification This is no longer so The advent of recombinant DNA technology a technology which includes the discovery of restriction endonucleases i e the ability to cut at specific DNA sites thus enabling us to cut out defined chunks of DNA the discovery of methods of inserting foreign DNA into other organisms and also of having them expressed in quantity has given biochemists a useful and general way of producing large quantities of almost any DNA RNA and protein they want to study It has also provided a way to produce modified enzymes on demand This makes it possible to test models for catalysis for example by making an enzyme that lacks a particular amino acid in the active site The experiments in the DNA section of the course cover some of the basic methods used in recombinant DNA work Your instructors have not been able to reduce the content of some of recombinant DNA protocols so that they can be completed in a single afternoon Therefore you may sometimes be required to come in early to get an



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