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VOLTAGE-PROGRAMMABLE BIOFUNCTIONALITY IN MEMS ENVIRONMENTS



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VOLTAGE PROGRAMMABLE BIOFUNCTIONALITY IN MEMS ENVIRONMENTS USING ELECTRODEPOSITION OF A REACTIVE POLYSACCHARIDE Li Qun Wu a b Hyunmin Yi a c Sheng Li d e David A Small a c Jung Jin Park f Gary W Rubloff d f Reza Ghodssi d e William E Bentley a c and Gregory F Payne a b a Center for Biosystems Research UMBI b Chemical and Biochemical Engineering UMBC c Chemical Engineering UMCP d Institute for Systems Research UMCP e Electrical and Computer Engineering UMCP f Materials and Nuclear Engineering UMCP Tel 301 405 8389 Fax 301 314 9075 Email payne umbi umd edu ABSTRACT EXPERIMENTAL The amino polysaccharide chitosan has distinct properties that make it an attractive interface material for the assembly of biomolecules onto microfabricated surfaces Chitosan has pH responsive electrostatic and solubility properties that allow it to be deposited and retained on cathode surfaces Deposition is shown to be spatially controllable at m levels by templating the chitosan onto micropatterned gold cathodes Temporal control of deposition can be achieved depending on when the micropatterned electrodes are polarized Chitosan also has nucleophilic amine groups that can be easily reacted using standard amine specific chemistries Studies show that the sequence of chitosan deposition and chemical modification is repeatable Finally standard chemistries can be exploited to couple biomolecules onto chitosan films that have been templated onto the micropatterened gold cathodes Specifically we used glutaraldehyde activation to assemble the model protein green fluorescent protein GFP onto a chitosan deposit These studies demonstrate that chitosan has unique properties that allow it to serve as an interface material for the assembly of biomolecules onto microfabricated surfaces INTRODUCTION The effective integration of biotechnology and microfabrication will provide robust biosensors to better diagnose disease rapidly detect chemical biological agents and efficiently discover drugs The biological



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