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Electrophoresis_Set_Up




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12/10/17 1 Electrophoresis The purpose of electrophoresis is to separate molecules of DNA, RNA or protein. Separation can be based upon: 1) Size 2) Shape 3) Isoelectric point (protein only). Separation is achieved by using a matrix (gel) and an electromotive force to propel the molecules through the gel . 12/10/17 2 Setting Up an Electrophoresis Gel • To run a gel electrophoresis you will need • Gel tray • Gel comb • Electrophoretic gel box • Agarose powder • Buffer solution • Dye • Electric field 12/10/17 19 Analyzing Your Gel • Turn off the power supply • Disconnect the wires • Remove the gel tray with the gel from the tray box • Stain gel with a dye that will bind to DNA (we recommend using Fast Blast DNA stain from Biorad) • Size DNA fragments using your DNA ladder Ladder Ladder DNA samples 12/10/17 20 Where to Get More Information Electronic sources • Dolan DNA Learning Center Electrophoresis Animation • Colorado State: Agarose Gel Electrophoresis of DNA • Mama Ji's Molecular Kitchen • Molecular Biology Cyberlab • Genetic Science Learning Center






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