BIOLOGY 263, FALL 2003 MIDTERM EXAMINATION Name Date: Laboratory Practical Stations. Answer the questions at each station. (12%, 4% per station) 1. a. 3. Complete the activity as directed. b. 2. a. b. Hypothesis Evaluation. Is there statistically significant support for each of the following hypotheses? (6%, 2% each) 1. Hypothesis: The number of insect species will be greater in grassland areas than in woodland areas in eastern Colorado. Data: Mean number of insect species per 1 meter quadrat in wooded areas = 22.4, Mean number of insect species per 1 meter quadrat in grassland areas = 18.8. p-value = 0.0050 Circle one: Hypothesis Supported Hypothesis NOT Supported 2. Hypothesis: Filed bindweed, Convolvulus arvensis, is more common empty lots than in lawns. Data = Mean # of Convolvulus arvensis plants per m2 in lawn = 18.5, Mean # of Convolvulus arvensis plants per m2 in empty lots = 25.2 p-value = 0.0592 Circle one: Hypothesis Supported Hypothesis NOT Supported 3. Hypothesis = There are more bacterial species in mowed areas than unmowed areas. Data = Mean # of bacterial species per 1g soil from mowed area on 1 plate of TSA nutrient medium = 10.4, Mean # of bacterial species per 1g soil from unmowed area on 1 plate of TSA nutrient medium = 9.1, p-value = 0.0480 Circle one: Hypothesis Supported Hypothesis NOT Supported Laboratory Safety & Behavior Scenario. Read the scenario below. List four things that Betty did incorrectly or unsafely and BRIEFLY (1 sentence) explain why each was incorrect/unsafe. (10%) Betty walks into lab wearing her new sandals after placing her morning coffee on the cart outside the door. She sits down and begins to work on the prokaryote laboratory with her laboratory partner. In collecting materials she lays a broth culture of Escherichia coli down on his lab bench. Mysteriously the culture begins to leak through its cap all over the bench. Betty reacts quickly and cleans the spill up using 95% ethanol and Kimwipes before anyone else noticed. She disposes of the used Kimwipes in the trash next to the sink. After that incident Betty is very careful completing the rest of the lab although it is hard for her to keep swinging her long hair away from the lit Bunsen burner since she is aware that this is something about which she should be careful.Definitions. Define each BIOLOGICAL term from THIS LABORATORY in an accurate, concise, and lucid manner. (20%, 4% each) 1. Bacterial Colony: 2. Electrophoresis: 3. Negative Control Group: 4. Quadrat: 5. Phylogenetic Tree: Data Manipulation & Analysis. Address each question in as concise and lucid a manner as possible. 1. Given the following data from a small, reproductively isolated, constant-sized population, a) calculate the allele and genotype frequencies for every time period. (8%) b) Create a properly labeled graph of the allele frequency of one of the alleles (NOT both) over time. (5%) c) Propose a plausible population genetic/evolutionary explanation for what you see happening (or not happening) to the allele frequency over time. (2%) individuals gener-ations BB Bb bb 0 6 12 6 5 4 14 6 10 8 14 2 15 1 10 13 20 0 0 242. Use the following four gels on which are shown X and x alleles (all individuals are from the same population) to calculate the allele and genotype frequencies for the population. Then determine if the population is at Hardy-Weinberg equilibrium. (10%) wells x X 2. Create a taxonomic key to the following organisms. (6%) Plancterus zebrinus ⇓ Catostomus commersoni ⇓ Fundulus chrysotus ⇓ Dorosoma cepedianum ⇓ Experimental Design. Use the back of this page to design a rigorous experiment to test the hypothesis that squirrel populations are more dense on the Regis campus than in the surrounding neighborhood. (13%) Create a scientific title for the laboratory research paper you would write for this experiment.