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Development of a Multianalyte Sensor on a Single Chip Sarah Jeffords Louisiana Tech University Dr Gerard Cot Rebecca Rounds This Research Experience for Undergraduates Site is Sponsored By Air Force Office of Scientific Research U S Air Force Department of Defense National Science Foundation NSF Grant No 0453578 Research Objectives Investigate sensing technology in hydrogel environment Determine analyte of interest i e pH oxygen Measure change with specific fluorophore i e BCECF ruthenium Determine biocompatible gel PEG with optimal porosity Determine proper mesh size of gel for analyte of interest Large enough to allow diffusion of analyte Small enough to contain the fluorophore Create and test sensor array across the following physiologically relevant conditions For oxygen 3 21 For pH 6 0 8 0 normal body pH is 7 4 Background Nonintrusive automated system for NASA Previous systems required manual measurement of sample Introduces possibility for contamination and human error Compact system suitable for small space allotment on shuttle Automatic system alleviates worry for time dependent analysis Monitors multiple analytes i e pH and oxygen with one sensing mechanism Poly ethylene glycol PEG hydrogel Cross linked structure biocompatible hydrophilic Mesh size can be altered to optimally contain fluorophore while allowing diffusion of analytes Microarray Sensor Fabrication Precursor Solution water PEG fluorophore darocur PDMS Mold Glass slide with TPM monolayer Photomask UV Light Microarray Sensor Optical Detection Setup Sensor Array Elements pH Results in PBS 1 4 1 2 PBS Room Temp PBS Cold Relative Intensity 1 0 8 0 6 0 4 0 2 0 6 6 5 7 Measured pH 7 5 8 pH Results in DMEM 1 4 1 2 DMEM Room Temp DMEM Cold Relative Intensity 1 0 8 0 6 0 4 0 2 0 6 6 5 7 Measured pH 7 5 8 Oxygen Results 1 2 PBS room PBS cold DMEM room DMEM cold Relative Intensity 1 0 8 0 6 0 4 0 2 0 0 21 Measured Oxygen Results and Conclusions For pH elements Increase in intensity with more basic pH Less



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