Quantitative Investigations of Hatching in Brine Shrimp Cysts




12 views

Unformatted text preview:

Quantitative Investigations of Hatching in Brine Shrimp Cysts Charlie Drewes Ecology Evolution Organismal Biology Iowa State University Ames IA Artemia franciscana North American brine shrimp Class Branchiopoda meaning gilled feet gilled feet crustacean subgroup e g fairy shrimp brine shrimp clam shrimp tadpole shrimp daphnids almost all freshwater use leaf like appendages to feed swim respire often produce anabiotic eggs Artemia fertilized eggs in brood sac life cycle release of encysted gastrula stage embryos winter eggs Adult female Artemia franciscana dry encysted winter eggs ASW Artemia X life cycle winter eggs HATCH Advantages of stuck on Artemia easy transfer handling of cysts quantitate starting of cysts and hatched follow development hatching of single cysts view hatching cysts in a single focal plane high rates of hatching survival no aeration required conservative use of cysts open ended investigations many variables Paint cysts on double stick tape with small soft dry paintbrush Method A Method A paint cysts on sticky circle Method B transparency strip with grid 10 mm x 10 mm strip of double stick tape covers grid plastic petri dish tape holds strip to dish Cysts painted on sticky grid with brush A1 A2 Encysted gastrulae in ASW and stuck to tape A1 Hatching of encysted Artemia embryos C1 B Cyst swells and wall splits A2 C2 C1 C2 Umbrella stage emerges membrane bound B D1 D2 Release of free swimming nauplius larvae D1 D2 Each day a pipet is used to remove and count hatched swimming nauplius larvae CONTROL Treatment Group control or experimental Initial number of cysts in dish 50 of hatched Cumulative Cumulative hatching cumulative of hatched larvae initial number of cysts X 100 nauplius larvae removed of hatched nauplius larvae day 1 2 3 Day 0 start 0 0 0 Day 1 24 h 22 22 44 Day 2 48 h 11 33 66 Day 3 72 h 6 39 78 Day 4 96 h 1 40 80 Typical hatching curve H A T C H Examples of experimental variables that may affect hatching curves 1 dry cysts exposed to






Loading Unlocking...
Login

Join to view Quantitative Investigations of Hatching in Brine Shrimp Cysts and access 3M+ class-specific study document.

or
We will never post anything without your permission.
Don't have an account?
Sign Up

Join to view Quantitative Investigations of Hatching in Brine Shrimp Cysts and access 3M+ class-specific study document.

or

By creating an account you agree to our Privacy Policy and Terms Of Use

Already a member?