I. NutritionA. Modes of growth (the “trophs”)1. Autotrophs2. Phototrophs3. Chemotrophs4. Combinationsa) Chemoheterotrophsb) Photoautotrophsc) Chemoautotrophs5. Obligate and facultative (Box 5.2)B. What does a cell need to grow?1. First let's see what cells are made of:2. Composition of cells (Table 5.1)3. C, O, N, H, S, P4. Salts5. Trace elements6. Growth factorsII. How do prokaryotes get their nutrients?A. By transporting it inB. Cytoplasmic membrane is selectively permeableC. Diffusion (Fig. 5.2)D. The role of active transport1. Secondary transporters (Fig. 5.4)a) Uniportersb) Symporters (Fig. 5.5)c) Antiporters2. Group translocation (Fig. 5.6)3. ABC Transporters (Fig. 5.3)a) Iron uptake example (Fig. 5.8)III. How to grow bacteria:A. Most prokaryotes cannot be cultured in the labB. Culture medium1. Complex2. Defined (Table 5.2, what’s missing?)3. Carbon source4. Energy source5. Solid vs. liquid6. Sterilization methods7. Aseptic techniquesC. Acidity and alkalinity1. Halophilic (Fig. 6.17)2. Acidiphilic vs. alkaliphilic3. HaloalkaliphilicD. Oxygen1. Aerobes (obligate/strict vs. facultative)2. Microaerophiles3. Anaerobes (obligate/strict vs. aerotolerant)4. Oxygen toxicity5. Anaerobic growth (Fig. 6.14)E. Temperature (Fig. 6.15 and 6.16)1. Cardinal temperatures2. Psychrophiles3. Mesophiles4. Thermophiles5. Hyperthermophiles6. Examples (Table 6.3)Lecture 5 Nutrition/Transport Bio119I. NutritionA. Modes of growth (the “trophs”)1. Autotrophs2. Phototrophs3. Chemotrophs4. Combinationsa) Chemoheterotrophsb) Photoautotrophsc) Chemoautotrophs5. Obligate and facultative (Box 5.2)B. What does a cell need to grow?1. First let's see what cells are made of:2. Composition of cells (Table 5.1)3. C, O, N, H, S, P4. Salts1Lecture 5 Nutrition/Transport Bio1195. Trace elements6. Growth factorsII. How do prokaryotes get their nutrients?A. By transporting it inB. Cytoplasmic membrane is selectively permeableC. Diffusion (Fig. 5.2)D. The role of active transport1. Secondary transporters (Fig. 5.4)a) Uniportersb) Symporters (Fig. 5.5)c) Antiporters2. Group translocation (Fig. 5.6)2Lecture 5 Nutrition/Transport Bio1193. ABC Transporters (Fig. 5.3)a) Iron uptake example (Fig. 5.8)III.How to grow bacteria:A. Most prokaryotes cannot be cultured in the labB. Culture medium1. Complex2. Defined (Table 5.2, what’s missing?)3. Carbon source4. Energy source5. Solid vs. liquid3Lecture 5 Nutrition/Transport Bio1196. Sterilization methods7. Aseptic techniquesC. Acidity and alkalinity1. Halophilic (Fig. 6.17)2. Acidiphilic vs. alkaliphilic3. HaloalkaliphilicD. Oxygen1. Aerobes (obligate/strict vs. facultative)2. Microaerophiles3. Anaerobes (obligate/strict vs. aerotolerant)4Lecture 5 Nutrition/Transport Bio1194. Oxygen toxicity5. Anaerobic growth (Fig. 6.14)E. Temperature (Fig. 6.15 and 6.16)1. Cardinal temperatures2. Psychrophiles3. Mesophiles4. Thermophiles5. Hyperthermophiles5Lecture 5 Nutrition/Transport Bio1196. Examples (Table
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