Recitation Section 8 March 2-3, 2005 Molecular Biology—Transcription and Translation Today we return to take another look at the yeast cystathionine beta synthase (CBS) protein. Below is the sequence of the yeast CBS gene and surrounding DNA. We will use this sequence for parts A and B of today’s section. The sequences of both strands of the DNA duplex are shown: the top strand reads 5' to 3' left to right (1 to 2040); the bottom, complimentary, strand reads 5' to 3' right to left (2040 to 1). 5’- CAACTTCACCCAAGTAAGGATAATCAGCTCTGTCGTGACTGATAAATGCTATATCCGGCA 1 ---------+---------+---------+---------+---------+---------+ 3’- GTTGAAGTGGGTTCATTCCTATTAGTCGAGACAGCACTGACTATTTACGATATAGGCCGT TATGCAGTCCACACGGCATTACCGTTTCACTAATTTATTGCCATCTTCCTCCACAGTTTT 61 ---------+---------+---------+---------+---------+---------+ ATACGTCAGGTGTGCCGTAATGGCAAAGTGATTAAATAACGGTAGAAGGAGGTGTCAAAA GCACCGAAAGGAAAAAAAGAAACCAACACCGAAAATTTTTTTCTCCTAAAGGTTAAAGTA 121 ---------+---------+---------+---------+---------+---------+ CGTGGCTTTCCTTTTTTTCTTTGGTTGTGGCTTTTAAAAAAAGAGGATTTCCAATTTCAT AACGCAAGGCACTTCACCAGGCTTGTATATATAAATGTCGTGATGCTTCTATGCCAAAGT 181 ---------+---------+---------+---------+---------+---------+ TTGCGTTCCGTGAAGTGGTCCGAACATATATATTTACAGCACTACGAACATACGGTTTCA AAAAGGCAACACTTGAAGATTTCGTTGTAGGCCACTTGCTCAAAGGACATCTAGATAAAT 241 ---------+---------+---------+---------+---------+---------+ TTTTCCGTTGTGAACTTCTAAAGCAACATCCGGTGAACGAGTTTCCTGTAGATCTATTTA ACGACGTAAGAATAAAAATGACTAAATCTGAGCAGCAAGCCGATTCAAGACATAACGTTA 301 ---------+---------+--------c+--------b+---------+---a-----+ TGCTGCATTCTTATTTTTACTGATTTAGACTCGTCGTTCGGCTAAGTTCTGTATTGCAAT TCGACTTAGTTGGTAACACCCCATTGATCGCACTGAAAAAATTGCCTAAGGCTTTGGGTA 361 ---------+----e----+--d------+---------+---------+---------+ AGCTCAATCAACCATTGTGGGGTAACTAGCGTGACTTTTTTAACGGATTCCGAAACCCAT TCAAACCACAAATTTATGCTAAGCTGGAACTATACAATCCAGGTGGTTCCATCAAAGACA 421 ---------+---------+---------+---------+---------+---------+ AGTTTGGTGTTTAAATACGATTCGACCTTGATATGTTAGGTCCACCAAGGTAGTTTCTGT GAATTGCCAAGTCTATGGTGGAAGAAGCTGAAGCTTCCGGTAGAATTCATCCTTCCAGAT 481 ---------+---------+---------+---------+---------+---------+ CTTAACGGTTCAGATACCACCTTCTTCGACTTCGAAGGCCATCTTAAGTAGGAAGGTCTA CTACTCTGATCGAACCTACTTCTGGTAACACCGGTATCGGTCTAGCTTTAATCGGCGCCA 541 ---------+---------+---------+---------+---------+---------+ GATGAGACTAGCTTGGATGAAGACCATTGTGGCCATAGCCAGATCGAAATTAGCCGCGGTTCAAAGGTTACAGAACTATCATCACCTTGCCGGAAAAAATGTCTAACGAGAAAGTTTCTG 601 ---------+---------+---------+---------+---------+---------+ AGTTTCCAATGTCTTGATAGTAGTGGAACGGCCTTTTTTACAGATTGCTCTTTCAAAGAC TCCTAAAGGCTCTGGGTGCTGAAATCATCAGAACTCCAACTGCTGCTGCCTGGGATTCTC 661 ---------+---------+---------+---------+---------+---------+ AGGATTTCCGAGACCCACGACTTTAGTAGTCTTGAGGTTGACGACGACGGACCCTAAGAG CAGAATCACATATTGGTGTTGCTAAGAAGTTGGAAAAAGAGATTCCTGGTGCTGTTATAC 721 ---------+---------+---------+---------+---------+---------+ GTCTTAGTGTATAACCACAACGATTCTTCAACCTTTTTCTCTAAGGACCACGACAATATG TTGACCAATATAACAATATGATGAACCCAGAAGCTCATTACTTTGGTACTGGTCGCGAAA 781 ---------+---------+---------+---------+---------+---------+ AACTGGTTATATTGTTATACTACTTGGGTCTTCGAGTAATGAAACCATGACCAGCGCTTT TCCAAAGACAGCTAGAAGACTTGAATTTATTTGATAATCTACGCGCTGTTGTTGCTGGTG 841 ---------+---------+---------+---------+---------+---------+ AGGTTTCTGTCGATCTTCTGAAGTTAAATAAAGTATTAGATGCGCGACAACAACGACCAC CTGGTACTGGTGGGACTATTAGCGGTATTTCCAAGTACTTGAAAGAACAGAATGATAAGA 901 ---------+---------+---------+---------+---------+---------+ GACCATGACCACCCTGATAATCGCCATAAAGGTTCATGAACTTTCTTGTCTTACTATTCT TCCAAATCGTTGGTGCTGACCCATTCGGTTCAATTTTAGCCCAACCTGAAAACTTGAATA 961 ---------f---------+---------+---------+---------+---------+ AGGTTTAGCAACCACGACTGGGTAAGCCAAGTTAAAATCGGGTTGGACTTTTGAACTTAT AGACTGATATCACTGACTACAAAGTTGAGGGTATTGGTTATGATTTTGTTCCTCAGGTTT 1021 ---------+---------+---------+------g--+---------+---------+ TCTGACTATAGTGACTGATGTTTCAACTCCCATAACCAATACTAAAACAAGGAGTCCAAA TGGACAGAAAATTAATTGATGTTTGGTATAAGACAGACGACAAGCCTTCTTTCAAATACG 1081 ---------+---------+---------+---------+---------+---------+ ACCTGTCTTTTAATTAACTACAAACCATATTCTGTCTGCTGTTCGGAAGAAAGTTTATGC CCAGACAATTGATTTCTAACGAAGGTGTCTTGGTGGGTGGTTCTTCCGGTTCTACCTTCA 1141 ---------+---------+---------+---------+---------+---------+ GGTCTGTTAACTAAAGATTGCTTCCACAGAACCACCCACCAAGAAGGCCAAGATGGAAGT CTGCGGTTGTGAAATACTGTGAAGACCACCCTGAACTGACTGAAGATGATGTCATTGTTG 1201 ---------+---------+---------+---------+---------+---------+ GACGCCAACACTTTATGACACTTCTGGTGGGACTTGACTGACTTCTACTACAGTAACAAC CCATATTCCCAGATTCCATCAGGTCGTACCTAACCAAATTCGTCGATGACGAATGGTTGA 1261 ---------+---------+---------+---------+---------+---------+ GGTATAAGGGTCTAAGGTAGTCCAGCATGGATTGGTTTAAGCAGCTACTGCTTACCAACT AAAAGAACAATTTGTGGGATGATGACGTGTTGGCCCGTTTTGACTCTTCAAAGCTGGAGG 1321 ---------+---------+---------+---------+---------+---------+ TTTTCTTGTTAAACACCCTACTACTGCACAACCGGGCAAAACTGAGAAGTTTCGACCTCC CTTCGACGACAAAATACGCTGATGTGTTTGGTAACGCTACTGTAAAGGATCTTCACTTGA 1381 ---------+---------+---------+---------+---------+---------+ GAAGCTGCTGTTTTATGCGACTACACAAACCATTGCGATGACATTTCCTAGAAGTGAACTAACCGGTTGTTTCCGTTAAGGAAACCGCTAAGGTCACTGATGTTATCAAGATATTAAAAG 1441 ---------+---------+---------+---------+---------+---------+ TTGGCCAAGAAAGGCAATTCCTTTGGCGATTCCAGTGACAACAATAGTTCTATAATTTTC ACAATGGCTTTGACCAATTGCCTGTGTTGACTGAAGACGGCAAGTTGTCTGGTTTAGTTA 1501 ---------+---------+---------+---------+---------+---------+ TGTTACCGAAACTGGTTAACGGACACAACTGACTTCTGCCGTTCAACAGACCAAATCAAT CTCTCTCTGAGCTTCTAAGAAAACTATCAATCAATAATTCAAACAACGACAACACTATAA 1561 ---------+---------+---------+---------+---------+---------+ GAGAGAGACTCGAAGATTCTTTTGATAGTTAGTTATTAAGTTTGTTGCTGTTGTGATATT AGGGTAAATACTTGGACTTCAAGAAATTAAACAATTTCAATGATGTTTCCTCTTACAACG 1621 ---------+---------+---------+---------+---------+---------+ TCCCATTTATGAACCTGAAGTTCTTTAATTTGTTAAAGTTACTACAAAGGAGAATGTTGC AAAATAAATCCGGTAAGAAGAAGTTTATTAAATTCGATGAAAACTCAAAGCTATCTGACT 1681 ---------+---------+---------+---------+---------+---------+ TTTTATTTAGGCCATTCTTCTTCAAATAATTTAAGCTACTTTTGAGTTTCGATAGACTGA TGAATCGTTTCTTTGAAAAAAACTCATCTGCCGTTATCACTGATGGCTTGAAACCAATCC 1741 ---------+---------+---------+---------+---------+---------+ ACTTAGCAAAGAAACTTTTTTTGAGTAGACGGCAATAGTGACTACCGAACTTTGGTTAGG ATATCGTTACTAAGATGGATTTACTGAGCTACTTAGCATAAATAAGAACCCACGCTTCAA 1801 ---------+---------+---------+---------+---------+---------+ TATAGCAATGATTCTACCTAAATGACTCGATGAATCGTATTTATTCTTGGGTGCGAAGTT ATAAAAGCAAACATAGAAGCAAAATCCGTCATTCCTTTCCTATTCAATTGCACCGTTCTC
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